Ricerca e articoli accademici sul preparato ImunoBran MGN-3

Modified Arabinoxylan Rice Bran (MGN-3/Biobran) Enhances Intracellular Killing of Microbes by Human Phagocytic Cells In Vitro PEER REVIEWED

M. Ghoneum, M. Matsuura and S. Gollapudi

(edited by Chris Gutch PhD.)

2008

Phagocytic cells, comprised of neutrophils and moncytyes/macrophages, play a key role in the innate immune response to infection. Our earlier study demonstrated that arabinoxylan rice bran (MGN-3/Biobran) activates murine peritoneal macrophage and macrophage cell lines. ln this study, we investigated whether MGN-3 can upregulate the phagocytic activity of human phagocytes in peripheral blood to phagocytize Escherichia coli (E. coli), trigger the oxidative burst and produce cytokines. Phagocytic cells were pre-labeled with dichlorofluorescin diacetate dye and were incubated with phycoerythrin-labeled. E. coli in thc presence or absence of MGN-3. Phagocytosis and oxidative burst were assessed by flow cytometry. Results showed that treatment with MGN-3 enhanced the phagocytosis of E. coli by neutrophils and moncytes. This was associated with an increased oxidative burst. ln addition, it caused a significant induction of cytokines (TNF-α, IL-6, IL-8 and lL—10); the effect was detected at 1 μg/ml and increased in a dose—dependent manner (P5 0.01). Notably, MGN-3 alone had no effect on the growth of 31 strains of bacteria suggesting that MGN-3 modulates phagocytic cellular function. These findings may have applications in the treatment of infections in the elderly and in immunocompromised patients.